Molecular Microbiology Laboratory - 1580 Words

Purpose To examine the microbial population of an elliptical at the Dixon Recreation Center by analysis of DNA from an uncultured community sample. 16S rRNA genes will distinguish bacteria and represent the microbial diversity of the sampled elliptical. An estimate of the cleanliness of the elliptical will be made based on species-abundance as well as evidence of any pathogenic strains. We will also compare and contrast respective populations of bacteria on clean and disinfected ellipticals to assess disinfectant effectiveness. Methods We collected bacteria from the elliptical, purified and amplified the total community DNA, and then analyzed the sequenced DNA with Mi Seq Software. A revised protocol was used to extract, purify, and†¦show more content†¦Discussion Sampled DNA came from an elliptical machine at the Dixon Recreation Center. The ellipticals are available to use for anyone working or studying at Oregon State University; there is a large population of people who regularly use these machines. The fitness equipment is potentially a rich source of bacteria and viruses. It is important to study the bacterial composition of the elliptical to reveal any pathogenic strains. Examining the microbial diversity of an elliptical will also provide an estimate the effectiveness of equipment cleaning procedures at Dixon Recreation Center. The PCR amplification of the community DNA was unsuccessful. There could be several reasons why no bands appeared in the gel, but I suspect that when we sampled the elliptical surface, the swath was not hydrated enough to effectively extract bacteria. Another possibility is that the surface was recently cleaned with antibacterial soap, which removed the majority of live bacteria, and helped to limit the amount of DNA available. We expected to see prominent bands in the gel, considering the sampled gym equipment has such heavy human traffic. The PCR product did not appear in the no-template negative control reaction. This was fortunate, as it’s easy to contaminate the reaction with foreign DNA during purification and amplification. The control functions to show thatShow MoreRelatedMolecular-Based Techniques for Diagnosing Microbial Infections1605 Words   |  7 PagesIntroduction Although some infections are unique enough to be identified clinically, usually microbiologic laboratory methods are needed to identify the etiologic agent and diagnose microbial infection (Washington, J.A., 1996). 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The Fire Sparked by the Buddhist Crisis - 3351 Words

The Fire Sparked by the Buddhist Crisis On June 11, 1963, Malcolm Browne took a photograph. His image would become the symbol for a period in the Vietnam conflict known as the Buddhist Crisis. Browne’s photo was of a monk sitting in the middle of the street, moments after dousing himself in fuel and lighting a match. The monk’s name was Thich Quang Duc, and his suicide would have repercussions not just in Vietnam but overseas in the United States as well. His action stimulated major growth in the Buddhist Crisis, inspiring leaders of the movement to increase militant actions against President Ngo Dinh Diem’s regime. Duc’s self-immolation was symbolic of something much larger than a single political act, representing a movement that would completely alter United States policy in Vietnam and eventually lead to the assassination of President Diem. Revered reporter David Halberstam wrote of Thich Quang Duc’s self-immolation in his book The Making of a Quagmire of, †¦a young Buddhist priest with a microphone saying calmly over and over again in Vietnamese and English, A Buddhist priest burns himself to death. A Buddhist priest becomes a martyr.† The fact that the priest was speaking in English showed that he wanted American reporters to get the story, he wanted Americans to understand what was occurring. The photograph captured a dramatic moment in time, but shared only a glimpse of the magnitude with which the Buddhist Crisis would affect the future of the VietnamShow MoreRelatedThe Era Of The Cold War Essay2000 Words   |  8 Pagesthe United States having a hand in supporting the government which held their ideals. John f. Kennedy had early involvement in the Vietnam war, supporting an unpopular government whose policy was the persecution Buddhists and many followers. Images of Buddhist monks setting themselves on fire turned public opinion against John f. Kennedy to leading support for this unpopular governm ent was withdrawn (Schaller 1095-1097). As a consequence of supporting and funding these questionable groups, AmericanRead More The Impact of Revolutions on the Cold War Essay2473 Words   |  10 Pagesof her Fire in the Lake that Ngo was very unpopular among the people he ruled over. Ngo faced opposition primarily from the NLF, which was an organization (which at first included communists and anti-communists) dedicated to the liberation of Vietnam from the domination of foreign government and the general amelioration of the conditions of the Vietnamese. The United States, however, condemned the NLF as a communist organization. Ngo came to be unaccepted by all after the Buddhist Crisis of 1963Read MoreEssay on Vietnam6962 Words   |  28 PagesII sparked violent confrontations with the French, culminating in the French military defeat at Dien Bien Phu in 1954. The Geneva Accords of 1954 temporarily divided Vietnam into two zones (the Communist north and the anti-Communist, US-supported south). Political and ideological opposition quickly turned to armed struggle, prompting the USA and other countries to commit combat troops in 1965. The Paris Peace Agreements, signed in 1973, provided an immediate cease-fire andRead MoreThe War Of The Vietnam War3899 Words   |  16 Pagesthe installment of military advisors in Vietnam, â€Å"Seeger composed ‘The Big Muddy’, a song about a dangerous river crossing during the Second World War, but one obviously aimed at the Johnson administration’s increasing involvement.† The song also sparked anger from television companies. An example of this was when Seeger was â€Å"banned†¦from performing his anti-war allegory, the song’s reference to the ‘big fool’ (i.e. Lyndon Johnson) was disrespectful.† Between 1963 and 1964, there was a significant

Leader On The Financial Performance Of Bank - Myassignmenthelp.Com

Question: Discuss about the Leader On The Financial Performance Of Bank. Answer: Mergers and acquisitions are explained as the consolidation of firms. Merger has been explained as the combination of two firms to one and on the other hand acquisition means taking over of one organization by another firm. The initiation of mergers and acquisitions leads to the increase in the value of the firm in comparison to their individual self. There have been numerous banks that have undergone mergers and acquisitions and one live example of a bank undertaking mergers and acquisitions has been Industrial Credit and Investment Corporations of India (ICICI) bank that functions mainly in India. The banks since its establishment have been performing in an effective manner and have been gaining profits. As the bank has been performing effectively, they have been acquiring various financial institutions and other banks. One of such mergers and acquisitions has been ICICI Bank acquiring Bank of Madura. The Bank of Madura over 57 years has been functioning as a profitable entity in the banking industry of India. The bank had key level of coverage in the southern areas of India. The bank has over 263 branches and the bank has a total asset of Rs 39.88 billion and deposit coverage of Rs 33.95 billion before the bank got merged. The bank had a capital adequacy ratio of 15.8%[1]. With the idea of enhancing their assets, demographic coverage and clientele base, ICICI Bank was looking for the mergers with private banks. Along with that with the rise in the cost of technological up gradation, required ICICI Bank to undertake certain mergers and acquisitions. Bank of Madura has an attractive figure of business per employee and extensive technological edge and therefore was an attraction for ICICI Bank even though issues related to human resource and cultural integration was significant. By looking at these aspects ICICI Bank declared the merger with Bank of Madura with its 263 beaches and various branches located in rural regions. On December 9th 2000, ICICI bank declared their merger with Bank of Madura and during this time Kotak Mahindra Group had stake of 26%, Spic group had about 4.7% and on the other hand UTI and LIC had marginal holdings. This merger has been found to raise the hold of ICICI Bank in the Southern sector of the country. The swap ratio was therefore agreed to be 1:2. This real life example of a bank undertaking mergers and acquisition has been useful for the completion of the paper. Capital Adequacy Ratio Capital/Year 2005-06 2006-07 2007-08 2008-09 Capital to risk-weighted asset ratio (CRAR) Tier-1 Capital (%) 9.20 7.42 11.76 12.16 Tier-2 Capital (%) 4.15 4.27 2.21 3.76 Ratio 13.35% 11.69% 13.97% 15.92% (Source:[3]) Reference "Cite A Website - Cite This For Me." Rspublication.com. N.p., 2017. Web. 18 Dec. 2017. "Personal Banking, Online Banking Services - ICICI Bank." Icicibank.com. N.p., 2017. Web. 18 Dec. 2017. Chandani, Arti, Mita Mehta, and K.B. Chandrasekaran. "A Working Paper On The Impact Of Gender Of Leader On The Financial Performance Of The Bank: A Case Of ICICI Bank." N.p., 2017. Print. [1] "Cite A Website - Cite This For Me." Rspublication.com. N.p., 2017. Web. 18 Dec. 2017. [2] "Personal Banking, Online Banking Services - ICICI Bank." Icicibank.com. N.p., 2017. Web. 18 Dec. 2017. [3] Chandani, Arti, Mita Mehta, and K.B. Chandrasekaran. "A Working Paper On The Impact Of Gender Of Leader On The Financial Performance Of The Bank: A Case Of ICICI Bank." N.p., 2017. Print.

King Lear Essays (575 words) - King Lear, British Films,

King Lear King Lear In the play King Lear written by William Shakespeare a collection of images are used to express different points Shakespeare is trying to relay to his audience. One reoccurring image that kept popping up was animal images. Shakespeare displays these animal images when King Lear and many of the other characters in the play talk about Goneril and Regan. The animals that Lear and the other characters compare the two sisters to are not very pretty. They are compared to the likes of tigers, serpents, and even monsters. These reoccurring images have an important idea behind them that Shakespeare hopes to communicate his readers. Shakespeare waste no time in comparing Goneril and Regan to animals. When Lear parts from Goneril at the end of Act I, after she has sneered at him and diminished the number of his retainers, he calls her a ?Detested kite? (I. iv. 269.). He also compares her to ?the sea-monster? (I. iv. 268.), by which he possibly means a mythological monster that would betray its own father. King Lear also comments on his daughters ingratitude using animal imagery when he said,? How sharper than a serpent's tooth it is to have a thankless child? (I. iv. 295-296.). Lear comments once more on his daughter's ?monster ingratitude? (I. v. 40.). Lear is showing how he feels about how his daughters are treating him by comparing them to unpleasant animals. Lear in scene IV has a quarrel with his other daughter, Regan, where again he uses animal images to show how his daughters are sinking below manhood to animals. Lear seeks out his daughter, Regan, at Gloucester's castle, and finds out that her husband has put his faithful friend Kent in the stocks and that both husband and wife have retired to bed and do not wish to see him. When Regan finally comes down, she tells him ?You should be ruled, and led by some discretion that discerns your state better than yourself? (II. iv. 147-149). Lear responds by saying ?struck me with her tongue, most serpentlike, upon the very heart. (II. iv. 159-160). Lear here again is describing Regan to a serpent, which is a large poisonous snake. Both daughters seem to him now like unusually cruel animals. They show this when they shut him out into the stormy night. In the storm scene, Lear's hurt from his daughters affect his attitude to the mad Tom of Bedlam (Edgar). He thinks, on the analogy of his own suffering, that his daughters must have abused Poor Tom. Nothing else could have brought him to such a pathetic state. This reminds Lear of his own ?pelican daughters? (III. iv. 75). This is an allusion to the medieval belief that pelican young fed on the blood of the parent bird. This analogy compares to how Lear's daughters are feeding on him giving them the control of the kingdom. Shakespeare uses these animal images throughout his play to describe Goneril and Regan. It will be noticed that most of the animals used in these comparisons are unpleasant (kite, serpent, tigers, pelicans, foxes, and even monsters). Shakespeare is showing that the sisters are sinking from the level of man, who stood between the angels and the animals, to the level of the animals. They have become like some of the most unpleasant birds and animals of prey. In their cruelty and unnaturalness they are less than human. Shakespeare Essays