Molecular Microbiology Laboratory - 1580 Words

Purpose To examine the microbial population of an elliptical at the Dixon Recreation Center by analysis of DNA from an uncultured community sample. 16S rRNA genes will distinguish bacteria and represent the microbial diversity of the sampled elliptical. An estimate of the cleanliness of the elliptical will be made based on species-abundance as well as evidence of any pathogenic strains. We will also compare and contrast respective populations of bacteria on clean and disinfected ellipticals to assess disinfectant effectiveness. Methods We collected bacteria from the elliptical, purified and amplified the total community DNA, and then analyzed the sequenced DNA with Mi Seq Software. A revised protocol was used to extract, purify, and†¦show more content†¦Discussion Sampled DNA came from an elliptical machine at the Dixon Recreation Center. The ellipticals are available to use for anyone working or studying at Oregon State University; there is a large population of people who regularly use these machines. The fitness equipment is potentially a rich source of bacteria and viruses. It is important to study the bacterial composition of the elliptical to reveal any pathogenic strains. Examining the microbial diversity of an elliptical will also provide an estimate the effectiveness of equipment cleaning procedures at Dixon Recreation Center. The PCR amplification of the community DNA was unsuccessful. There could be several reasons why no bands appeared in the gel, but I suspect that when we sampled the elliptical surface, the swath was not hydrated enough to effectively extract bacteria. Another possibility is that the surface was recently cleaned with antibacterial soap, which removed the majority of live bacteria, and helped to limit the amount of DNA available. We expected to see prominent bands in the gel, considering the sampled gym equipment has such heavy human traffic. The PCR product did not appear in the no-template negative control reaction. This was fortunate, as it’s easy to contaminate the reaction with foreign DNA during purification and amplification. The control functions to show thatShow MoreRelatedMolecular-Based Techniques for Diagnosing Microbial Infections1605 Words   |  7 PagesIntroduction Although some infections are unique enough to be identified clinically, usually microbiologic laboratory methods are needed to identify the etiologic agent and diagnose microbial infection (Washington, J.A., 1996). 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